Reporter

Part:BBa_K1467400:Design

Designed by: Cara Deal, Jessica Gray   Group: iGEM14_NRP-UEA-Norwich   (2014-09-24)


RFP coding device - Golden Gate Module Flipper


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 793
    Illegal AgeI site found at 905
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1082
    Illegal BsaI.rc site found at 6


Design Notes

The sequences inserted (in orange) between the RFP and the BioBrick prefix and suffix include an inverted pair of BsaI recognition sequences that release the RFP to provide 4bp 5' overhangs compatible with the GoldenGate MoCloassembly standard, directly adjacent to the BioBrick suffix.


Source

BBa_J04450

References